Comparison of multiplex real-time PCR and ergosterol assays in quantifying Heterobasidion annosum in planta

A.M. Hietala, M. Eikenes, M. Kvaalen, H. Solheim and C.G. Fossdal
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A.M. Hietala: Norwegian Forest Research Institute, 1432 Ås, Norway *
M. Eikenes: Norwegian Forest Research Institute, 1432 Ås, Norway *
M. Kvaalen: Norwegian Forest Research Institute, 1432 Ås, Norway *
H. Solheim: Norwegian Forest Research Institute, 1432 Ås, Norway *
C.G. Fossdal: Norwegian Forest Research Institute, 1432 Ås, Norway *

Plant Protection Science, 2002, vol. 38, issue SI2-6thConfEFPP, 406-407

Abstract: A quantitative multiplex real-time PCR procedure was developed to monitor the dynamics in Norway spruce-Heterobasidion annosum pathosystem. The assay reliably detected down to 1 pg of H. annosum DNA and 1 ng of host DNA in multiplex conditions. As a comparative method for quantifying fungal colonization, we applied the ergosterol assay. There was a very high correlation between the results obtained with the two methods, this strengthening the credibility of both assays. The advantages and disadvantages of these assays are discussed.

Keywords: Heterobasidion; Norway spruce; infection; quantification (search for similar items in EconPapers)
Date: 2002
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10507-pps

DOI: 10.17221/10507-PPS

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