Promoter studies of chemically induced BCI-genes in the pathosystem barley - powdery mildew

U. Geldermann, G. Langen and K.-H. Kogel
Additional contact information
U. Geldermann: Institute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, 35392 Giessen, Germany
G. Langen: Institute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, 35392 Giessen, Germany
K.-H. Kogel: Institute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, 35392 Giessen, Germany

Plant Protection Science, 2002, vol. 38, issue SI2-6thConfEFPP, 487-488

Abstract: Chemical resistance inducers like BTH (S-methyl benzo (1,2,3)-thiadiazole-7-carbothiate) and DCINA (2,6-dichloro isonicotinic acid) activate resistance in barley against powdery mildew (Blumeria graminis f.sp. hordei). Nine BTH induced genes (Bci, barley chemically induced) have been identified in barley (BESSER et al. 2000) which are not responsive to pathogens in contrast to PR-proteins. From two Bci-genes (Bci3: similar to vsp, Bci4: Ca2+-binding EF-hand protein), the promoters were isolated. In transient transformation assays using promoter::GFP and promoter::GUS-constructs the functionality of these chemically induced promoters were studied. To identify the minimal promoter and regions with regulatory elements 5'-deletion constructs were used. Additionally, gel mobility shift assays were performed.

Keywords: barley; promoter; Bci; BTH; SAR (search for similar items in EconPapers)
Date: 2002
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pps.agriculturejournals.cz/doi/10.17221/10531-PPS.html (text/html)
http://pps.agriculturejournals.cz/doi/10.17221/10531-PPS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10531-pps

DOI: 10.17221/10531-PPS

Access Statistics for this article

Plant Protection Science is currently edited by Ing. Eva Karská, (Executive Editor PPS)

More articles in Plant Protection Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().