Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging

A. Chini, S. Murray, J. Grant, C. Thomson and G. Loake
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A. Chini: Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *
S. Murray: Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *
J. Grant: Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *
C. Thomson: Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *
G. Loake: Institute of Cell & Molecular Biology, University of Edinburgh, Scotland, United Kingdom; Daniel Rutherford Building, King's Buildings', Edinburgh EH9 3JH, Scotland, United Kingdom *

Plant Protection Science, 2002, vol. 38, issue SI2-6thConfEFPP, 615-616

Abstract: In order to identify components of the defence signalling network that may contribute to the establishment of disease resistance, we generated a novel PR-1::Luciferase transgenic line which was deployed in an imaging based screen to uncover novel defence-related mutants. Approximately, 5000 ethylmethane sulfonate (EMS) lines and 30 000 activation tagged lines were generated and screened for enhanced LUC activity via ultra low light imaging.

Keywords: signal transduction; luciferase imaging; systemic acquired resistance; Arabidopsis mutants (search for similar items in EconPapers)
Date: 2002
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:38:y:2002:i:si2-6thconfefpp:id:10570-pps

DOI: 10.17221/10570-PPS

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