Identification of Xiphinema vuittenezi by polymerase chain reaction

Shesh Kumari, Jiban K. Kundu and Jaroslav Polák
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Shesh Kumari: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
Jiban K. Kundu: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
Jaroslav Polák: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

Plant Protection Science, 2004, vol. 40, issue 1, 1-4

Abstract: So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies. Therefore, a polymerase chain reaction (PCR) protocol was optimised that both reliably and rapidly identifies X. vuittenezi. The internal transcribed spacer (ITS) species-specific primer of ribosomal DNA gene of X. vuittenezi was used. Nine populations of this species from Central Bohemia were investigated by means of PCR.

Keywords: nematodes; Xiphinema vuittenezi; PCR; ITS primer (search for similar items in EconPapers)
Date: 2004
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:40:y:2004:i:1:id:3120-pps

DOI: 10.17221/3120-PPS

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