Management of bacterial blight of cotton using a mixture of Pseudomonas fluorescens and Bacillus subtilis

Khabbaz Salaheddin, Veeramuthu Valluvaparidasan, Duraisamy Ladhalakshmi and Rethinasamy Velazhahan
Additional contact information
Khabbaz Salaheddin: General Commission for Scientific Agricultural Research, Hama, Syria
Veeramuthu Valluvaparidasan: Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
Duraisamy Ladhalakshmi: Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
Rethinasamy Velazhahan: Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

Plant Protection Science, 2010, vol. 46, issue 2, 41-50

Abstract: The potential of antagonistic rhizobacteria in the management of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum (Xam) was evaluated under greenhouse and field conditions. In this study, 93 bacterial isolates from the rhizosphere of cotton were screened for their efficacy in inhibiting the growth of Xam in vitro. Among them, 21 isolates were found to inhibit the in vitro growth of Xam. These isolates were identified as Pseudomonas fluorescens and Bacillus subtilis based on phenotypic characteristics, biochemical properties and using 16S-23S intergenic transcribed spacer-Polymerase Chain Reaction (PCR). Among the 21 isolates, the isolates P. fluorescens Pf32 and P. fluorescens Pf93 and B. subtilis B49 exhibited the maximum inhibitory activity against Xam. Talc-based powder formulations of the effective antagonistic isolates of P. fluorescens (Pf32, Pf93) and B. subtilis (B49) were developed and evaluated individually and in combination for their efficacy in the management of bacterial blight of cotton under greenhouse and field conditions. The P. fluorescens isolates Pf32 and Pf93 and Bacillus subtilis isolate B49 survived well in the talc-based formulation for more than 90 days. The application of a mixture of Pf32, Pf93 and B49 to seed, soil and foliage significantly reduced the bacterial blight incidence and increased the plant height, number of branches and number of bolls under field conditions. The plots treated with a mixture of Pf32, Pf93 and B49 recorded the maximum yield of 1915 kg/ha and 1512 kg/ha in trial I and trial II compared to 1210 kg/ha and 987 kg/ha in the untreated control, respectively.

Keywords: Keywords: biological control; Pseudomonas fluorescens; Bacillus subtilis; Gossypium hirsutum; bacterial blight (search for similar items in EconPapers)
Date: 2010
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pps.agriculturejournals.cz/doi/10.17221/20/2009-PPS.html (text/html)
http://pps.agriculturejournals.cz/doi/10.17221/20/2009-PPS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:46:y:2010:i:2:id:20-2009-pps

DOI: 10.17221/20/2009-PPS

Access Statistics for this article

Plant Protection Science is currently edited by Ing. Eva Karská, (Executive Editor PPS)

More articles in Plant Protection Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().