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Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

Ali Darvishzadeh, Ali Reza Bandani and Seyed Qasem Mousavi
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Ali Darvishzadeh: Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Ali Reza Bandani: Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Seyed Qasem Mousavi: Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Plant Protection Science, 2014, vol. 50, issue 2, 84-89

Abstract: We identify and characterise α-amylases of the two aphid species, A. fabae and A. gossypi. To do this, α-amylases of the two insect species were extracted and their activities were determined using 1% soluble starch. Results showed that α-amylase, which hydrolyses starch, is present in both aphids. Also, it was shown that optimum pH and temperature for the α-amylases of both species is 7.0 and 40°C, respectively. Gel assays using zymogram analysis showed that in both aphid species more than one isoform (two isoforms) of α-amylases hydrolyszing carbohydrates are present.

Keywords: aphids; gel assay; starch (search for similar items in EconPapers)
Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:50:y:2014:i:2:id:71-2012-pps

DOI: 10.17221/71/2012-PPS

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