Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture

Josef Špak, Daniela Pavingerová, Jaroslava Přibylová, Vlastimila Špaková, František Paprštein and Jiří Sedlák
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Josef Špak: Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Daniela Pavingerová: Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Jaroslava Přibylová: Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Vlastimila Špaková: Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
František Paprštein: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic
Jiří Sedlák: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic

Plant Protection Science, 2014, vol. 50, issue 4, 174-178

Abstract: In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2-3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50-60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.

Keywords: BRRV; in vitro; PCR; Vaccinium corymbosum L.; virus-free; virus elimination (search for similar items in EconPapers)
Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:50:y:2014:i:4:id:84-2013-pps

DOI: 10.17221/84/2013-PPS

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