Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Tara Solgi, Mehdi Moradyar, Mohammad Reza Zamani and Mostafa Motallebi
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Tara Solgi: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Mehdi Moradyar: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Mohammad Reza Zamani: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Mostafa Motallebi: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Plant Protection Science, 2015, vol. 51, issue 1, 6-12

Abstract: An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in canola (R line Hyola 308). Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens. The insertion of the transgene was verified by PCR and Southern blotting. The transgenic over-expression approach was used in order to investigate antifungal activity of expressed Chit33 on Sclerotinia sclerotiorum. Antifungal activity was detected in transgenic canola using detached leaf assay. Lesion sizes of transgenic canola caused by S. sclerotiorum were significantly retarded when compared to non-transgenic canola plant.

Keywords: antifungal activity; Brassica napus; chit33-cDNA; fungal diseases (search for similar items in EconPapers)
Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:51:y:2015:i:1:id:83-2013-pps

DOI: 10.17221/83/2013-PPS

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