Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques

Jiří Sedlák, František Paprštein, Josef Korba and Jana Šillerová
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Jiří Sedlák: Research and Breeding Institute of Pomology Holovousy Ltd., Hořice, Czech Republic
František Paprštein: Research and Breeding Institute of Pomology Holovousy Ltd., Hořice, Czech Republic
Josef Korba: Division of Crop Protection and Plant Health, Department of Phytobacteriology, Crop Research Institute, Prague, Czech Republic
Jana Šillerová: Division of Crop Protection and Plant Health, Department of Phytobacteriology, Crop Research Institute, Prague, Czech Republic

Plant Protection Science, 2015, vol. 51, issue 1, 1-5

Abstract: The suitability of different in vitro artificial inoculation methods for testing apple resistance to fire blight was evaluated. Six proliferation MS media containing BAP, TDZ or 2iP were tested to determine favourable conditions for shoot development. Out of the tested cytokinins, only media with BAP proved to be useful for all tested cultivars for artificial inoculation with Erwinia amylovora. Out of three tested inoculation methods, removal of 3 mm of the shoot apex with scissors and transfer of bacterial suspension by pipette (method 1) appeared to be the most suitable technique. This method enabled the determination of susceptibility of two cultivars due to the different speed of fire blight symptom development.

Keywords: fire blight; Malus L.; cultivar; artificial inoculation; medium; growth regulator (search for similar items in EconPapers)
Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:51:y:2015:i:1:id:94-2013-pps

DOI: 10.17221/94/2013-PPS

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