Cherry necrotic rusty mottle and Cherry green ring mottle viruses in Czech cherry germplasm

Josef Špak, Jaroslava Přibylová, Dana Šafářová, Ondřej Lenz, Igor Koloniuk, Milan Navrátil, Jana Fránová, Vlastimila Špaková and František Paprštein
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Jaroslava Přibylová: Institute of Plant Molecular Biology, Biology Centre v.v.i., Czech Academy of Sciences, České Budějovice, Czech Republic
Dana Šafářová: Faculty of Science, Palacky University, Olomouc, Czech Republic
Ondřej Lenz: Institute of Plant Molecular Biology, Biology Centre v.v.i., Czech Academy of Sciences, České Budějovice, Czech Republic
Igor Koloniuk: Institute of Plant Molecular Biology, Biology Centre v.v.i., Czech Academy of Sciences, České Budějovice, Czech Republic
Milan Navrátil: Faculty of Science, Palacky University, Olomouc, Czech Republic
Jana Fránová: Institute of Plant Molecular Biology, Biology Centre v.v.i., Czech Academy of Sciences, České Budějovice, Czech Republic
Vlastimila Špaková: Institute of Plant Molecular Biology, Biology Centre v.v.i., Czech Academy of Sciences, České Budějovice, Czech Republic
František Paprštein: Research and Breeding Institute of Pomology Ltd., Hořice, Czech Republic

Plant Protection Science, 2017, vol. 53, issue 4, 195-200

Abstract: Using reverse transcription polymerase chain reaction, 160 sweet and sour cherry trees from a germplasm collection, orchards, and wild trees in the Czech Republic were screened for the presence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV). The viruses were detected exclusively in sweet cherry trees in the germplasm collection, with CNRMV determined in two trees and CGRMV in four trees. Using next-generation sequencing, nearly complete genomic sequences (complete ORFs) were obtained for one CNRMV and three CGRMV isolates. Their relatedness to GenBank sequences of isolates from different countries together with negative results from screening outside of the germplasm collection suggests that the viruses had been imported with accessions.

Keywords: CNRMV; CGRMV; next-generation sequencing; Prunus avium; Prunus cerasus; detection (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:53:y:2017:i:4:id:160-2016-pps

DOI: 10.17221/160/2016-PPS

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