PCR-based diagnostic methods for 'Candidatus Liberibacter solanacearum' - Review

Seyed Mahyar Mirmajlessi, Maria Jennifer Sjölund, Marika Mänd, Marianne Loiseau, Vincenza Ilardi, Geert Haesaert, Reet Karise, Richard Alexander Gottsberger, Jason Sumner-Kalkun and Assunta Bertaccini
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Seyed Mahyar Mirmajlessi: Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
Maria Jennifer Sjölund: Science and Advice for Scottish Agriculture (SASA), Edinburgh, UK
Marika Mänd: Department of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia
Marianne Loiseau: French Agency for Food, Environmental and Occupational Health and Safety, Angers, France
Vincenza Ilardi: Centro Difesa e Certificazione, CREA, Rome, Italy
Geert Haesaert: Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
Reet Karise: Department of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, Estonia
Richard Alexander Gottsberger: Department for Molecular Diagnostics of Plant Diseases, Institute for Sustainable Plant Production, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria
Jason Sumner-Kalkun: Science and Advice for Scottish Agriculture (SASA), Edinburgh, UK
Assunta Bertaccini: Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy

Plant Protection Science, 2019, vol. 55, issue 4, 229-242

Abstract: 'Candidatus Liberibacter solanacearum' is an economically important pathogen in the Americas, New Zealand and Europe. The primary objective of this review is to systematically investigate the polymerase chain reaction (PCR)-based methods used for its detection in plant samples. Several databases were searched from the inception of the relevant literature up to August 2018. This review identified 53 studies that met all the inclusion criteria. The performance of the different methods was also compared, however due to data heterogeneity and insufficient evidence on the sensitivity of all assays used, a meta-analysis of the data was not possible. Nonetheless, the review indicates that the rtPCR designed to the 16S ribosomal RNA gene can be routinely employed as a fast, cost-effective, and reliable detection technique in diagnostic laboratories.

Keywords: Lso; leaf stunting; early detection; identification; in planta; molecular methods (search for similar items in EconPapers)
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:55:y:2019:i:4:id:145-2018-pps

DOI: 10.17221/145/2018-PPS

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