First report of 'Candidatus Phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis L.)

Mustafa Usta, Abdullah Güller and Hikmet Murat Sipahioglu
Additional contact information
Mustafa Usta: Department of Plant Protection, Faculty of Agriculture, Yuzuncu Yil University, Van, Turkey
Abdullah Güller: Department of Plant Protection, Faculty of Agriculture, Bingöl University, Bingöl, Turkey
Hikmet Murat Sipahioglu: Department of Plant Protection, Faculty of Agriculture, Malatya Turgut Özal University, Malatya, Turkey

Plant Protection Science, 2021, vol. 57, issue 3, 188-195

Abstract: The natural occurrence of 'Candidatus Phytoplasma trifolii' in pear trees (Pyrus communis Linnaeus) is reported here for the first time. In 2017, a total of thirty-five pear trees, two of them exhibiting leaf rolling along the midvein, reddening, bushy appearance, and upright growth symptoms were sampled in different locations in Van province, Turkey. The total deoxyribonucleic acid was extracted from symptomatic and asymptomatic plants. The purified DNA served as a template in nested polymerase chain reaction (nested-PCR) assays, performed to amplify 16S rRNA sequences using universal primer pairs (R16mF2/R16mR1 and R16F2n/R16R2). The resulting PCR products were then cloned into a pGEM T-Easy vector and sequenced bidirectionally. The phytoplasma strain, group, and subgroup identity were determined using the in silico restriction fragment length polymorphism (RFLP) analysis of the 16S ribosomal RNA-encoding gene sequences profiling with seventeen distinct restriction enzymes. Of the thirty-five pear samples, only two yielded 1 256 bp and 1 258 bp DNA fragments and were designated as Van-Pr3 (Acc. No. MH709141) and Van-Pr4 (Acc. No. MH730561), respectively. Based on the in silico virtual RFLP pattern analysis of the 16S rRNA sequences, we confirmed the presence of 'Ca. P. trifolii' belonging to the clover proliferation group and both identified phytoplasmas were identical with the similarity coefficient of 1.00 to the reference pattern of 16Sr group VI, subgroup A (Acc. No. AY390261). Here we report that the pear tree is an alternate host of the 'Ca. P. trifolii'.

Keywords: clover proliferation group; detection; new host; pear; nested-PCR; virtual RFLP; Turkey (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
http://pps.agriculturejournals.cz/doi/10.17221/163/2020-PPS.html (text/html)
http://pps.agriculturejournals.cz/doi/10.17221/163/2020-PPS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:57:y:2021:i:3:id:163-2020-pps

DOI: 10.17221/163/2020-PPS

Access Statistics for this article

Plant Protection Science is currently edited by Ing. Eva Karská, (Executive Editor PPS)

More articles in Plant Protection Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().