Characterization of an isolate of Lettuce big-vein associated virus (LBVaV) detected in naturally infected tomato (Solanum lycopersicum L.) in Slovakia

Jana Tomašechová, Lukáš Predajňa, Daniel Mihálik, Michaela Mrkvová, Pavel Cejnar, Katarína Šoltys, Sead Sabanadzovic and Miroslav Glasa
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Jana Tomašechová: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic
Lukáš Predajňa: Biomedical Research Center of the Slovak Academy of Sciences, Institute of Virology, Bratislava, Slovak Republic
Daniel Mihálik: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic
Michaela Mrkvová: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic
Pavel Cejnar: Department of Computing and Control Engineering, Faculty of Chemical Engineering, University of Chemistry and Technology in Prague, Prague, Czech Republic
Katarína Šoltys: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic
Sead Sabanadzovic: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, USA
Miroslav Glasa: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic

Plant Protection Science, 2021, vol. 57, issue 4, 344-348

Abstract: A tomato plant (Solanum lycopersicum Linnaeus, labelled KVE) displaying virus-like symptoms, tested negative for common tomato viruses, was subjected to high-throughput sequencing (HTS) on the Illumina MiSeq platform using ribosomal RNA-depleted total RNA as a template. The analysis has revealed the contigs mapping to Lettuce big-vein associated virus (LBVaV). The near complete LBVaV-KVE sequence of RNA1 and RNA2 revealed 95.0 and 94.9% identity with the reference sequence, the same length of translated products and a typical varicosavirus genome organisation. After initial long-term maintenance of LBVaV-KVE in the original plant, the virus could be detected by RT-PCR or nanoLC-ESI-Q-TOF in new plants generated from lateral shoot cuttings or inoculated by stem chips, although not uniformly. So far, LBVaV was reported to infect lettuce and related species. Our study expands the natural host range of the LBVaV to tomato.

Keywords: genome; next generation sequencing; Solanaceae; varicosavirus (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:57:y:2021:i:4:id:56-2021-pps

DOI: 10.17221/56/2021-PPS

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