Identification and functional analysis of glyoxal oxidase gene from rubber tree anthracnose

Xuemei Xian, Kunmeng Li, Hui Liu, Zhenhui Wang, Kun Yuan, Yiyu Hu, Chengtian Feng, Meng Wang, Chunhua Lin, Qiguang He and Yu Zhang
Additional contact information
Xuemei Xian: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Kunmeng Li: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Hui Liu: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Zhenhui Wang: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Kun Yuan: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Yiyu Hu: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Chengtian Feng: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Meng Wang: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Chunhua Lin: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Qiguang He: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
Yu Zhang: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China

Plant Protection Science, 2023, vol. 59, issue 4, 337-347

Abstract: Glyoxal oxidase (GLOX, EC 1.2.3.15) is a class of hydrogen peroxide synthases involved in lignin degradation. Several GLOX genes of biotrophic and necrotrophic phytopathogenic fungi have been confirmed to contribute to fungal growth, development, and pathogenicity. However, the composition, sequence characteristics and functions of genes in the GLOX family of hemi-biotrophic phytopathogenic fungi remain poorly understood. This study identified a GLOX-encoding gene CsGLOX in the hemi-biotrophic phytopathogenic fungus Colletotrichum siamense by bioinformatics analysis and PCR cloning. The encoded protein contains a signal peptide and five cell wall stress-responsive component (WSC) domains at the N terminus, followed by a catalytic structural domain (AA5_1). CsGLOX-deficient mutants were constructed by homologous replacement, which significantly increased the formation of conidia and reduced the pathogenicity and the high-osmotic stress tolerance of the fungus. However, the deletions did not influence fungal filamentous growth. This study proposes that the CsGLOX gene is involved in pathogen conidial formation, pathogenicity, and osmotic stress response, which deepens our understanding of the pathogenic mechanism of hemi-biotrophic phytopathogenic fungus C. siamense.

Keywords: glyoxal oxidase; Colletotrichum siamense; pathogenicity; stress responses; pathogenic mechanism (search for similar items in EconPapers)
Date: 2023
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pps.agriculturejournals.cz/doi/10.17221/52/2023-PPS.html (text/html)
http://pps.agriculturejournals.cz/doi/10.17221/52/2023-PPS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:59:y:2023:i:4:id:52-2023-pps

DOI: 10.17221/52/2023-PPS

Access Statistics for this article

Plant Protection Science is currently edited by Ing. Eva Karská, (Executive Editor PPS)

More articles in Plant Protection Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().