Analysis of chromosome termini in potato varieties

J. Fajkus, M. Novotná and J. Ptáček
Additional contact information
J. Fajkus: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
M. Novotná: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
J. Ptáček: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Plant, Soil and Environment, 2002, vol. 48, issue 11, 477-479

Abstract: Chromosomes of potato (Solanum tuberosum) are terminated by telomeres, which are formed by tandemly repeated [TTTAGGG]n oligonucleotide sequence. The total length of blocks of telomeric DNA has been known to vary largely among plant species and their varieties, and also among individual chromosome arms within a single nucleus. To check for such differences in potato varieties, which could be of a possible use in genotyping, we performed pulsed-field gel electrophoretic analysis of terminal restriction fragments in selected potato varieties. We found a typical range of telomere lengths of 20-60 kb in most analysed varieties. In one of analysed varieties (Monalisa), telomeres of wider span (up to 80 kb) have been observed. Most of restriction enzymes (PvuII, HaeIII, TaqI) produced a resulting smeared hybridisation pattern of telomeres. When using BglII, however, a doublet hybridisation band could be observed. This may reflect differences in composition of telomere-associated sequences at different chromosome ends.

Keywords: plant chromosomes; telomere length; potato varieties; terminal restriction fragments (search for similar items in EconPapers)
Date: 2002
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pse.agriculturejournals.cz/doi/10.17221/4398-PSE.html (text/html)
http://pse.agriculturejournals.cz/doi/10.17221/4398-PSE.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:48:y:2002:i:11:id:4398-pse

DOI: 10.17221/4398-PSE

Access Statistics for this article

Plant, Soil and Environment is currently edited by Kateřina Součková

More articles in Plant, Soil and Environment from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().