Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

P. Vejl, S. Skupinová, P. Sedlák and J. Domkářová
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P. Vejl: Czech University of Agriculture in Prague, Czech Republic
S. Skupinová: Czech University of Agriculture in Prague, Czech Republic
P. Sedlák: Czech University of Agriculture in Prague, Czech Republic
J. Domkářová: Czech University of Agriculture in Prague, Czech Republic

Plant, Soil and Environment, 2002, vol. 48, issue 11, 486-489

Abstract: Progressive methods of molecular analyses of DNA are routinely used in the fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described in this article. European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown pathotype from Kašperské hory (K) locality were used. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1-Rro1 was product 411 bp detected (only in cause G. rostochiensis), by using of set Fpa2-Rpa1 the product 239 bp was detected (only G. pallida). For these reasons the identity of the European populations was confirmed. Cysts of population K were identified as G. pallida.

Keywords: Globodera rostochiensis; Globodera pallida; ITS-1 region; PCR; DNA markers; species identification (search for similar items in EconPapers)
Date: 2002
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:48:y:2002:i:11:id:4400-pse

DOI: 10.17221/4400-PSE

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