Evaluation of genetic diversity of Brassica napus germplasm from China and Europe assessed by RAPD markers

S.W. Hu, J. Ovesná, L. Kučera, V. Kučera and M. Vyvadilová
Additional contact information
S.W. Hu: Shaanxi Academy of Agricultural Sciences, Yangling, Shaanxi, China
J. Ovesná: Shaanxi Academy of Agricultural Sciences, Yangling, Shaanxi, China
L. Kučera: Shaanxi Academy of Agricultural Sciences, Yangling, Shaanxi, China
V. Kučera: Shaanxi Academy of Agricultural Sciences, Yangling, Shaanxi, China
M. Vyvadilová: Shaanxi Academy of Agricultural Sciences, Yangling, Shaanxi, China

Plant, Soil and Environment, 2003, vol. 49, issue 3, 106-113

Abstract: The genetic diversity and the relationships among rapeseed germplasm, including a collection of 20 Chinese, 25 Czech, 2 German, 2 French, and 1 English cultivars and breeding materials were evaluated using random amplified polymorphic DNA (RAPD) markers. A total of 79 different polymorphic amplification products were obtained using10 selected decamer primers. RAPDs revealed a significant level of polymorphism among the accessions. The diversity index (DI) ranged from 1.390 to 3.491, showing a sufficient potential of selected primers to differentiate among studied genotypes. Three different metrics were used to assess genetic diversity. The best fit between a priori knowledge about germplasm origin and a posteriori grouping was found using Hamman metrics. Cluster analysis based on Hamman pairwise distance comparison divided the studied accessions into three main clusters. The first group included only accessions fromChina, the second group only that fromEurope with the exception of Zhongshuang No. 2, a Chinese winter rape possessing European cultivars in the pedigree. The third group included accessions both fromChina andEurope. The results indicate the occurrence of a considerable genetic variation between Chinese and European accessions.

Keywords: Brassica napus; RAPD; genetic diversity; genetic variability (search for similar items in EconPapers)
Date: 2003
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
http://pse.agriculturejournals.cz/doi/10.17221/4098-PSE.html (text/html)
http://pse.agriculturejournals.cz/doi/10.17221/4098-PSE.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:49:y:2003:i:3:id:4098-pse

DOI: 10.17221/4098-PSE

Access Statistics for this article

Plant, Soil and Environment is currently edited by Kateřina Součková

More articles in Plant, Soil and Environment from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().