Cot-based cloning and sequencing of the short arm of wheat chromosome 1B

H. Šimková, J. Janda, E. Hřibová, J. Šafář and J. Doležel
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H. Šimková: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic
J. Janda: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic
E. Hřibová: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic
J. Šafář: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic
J. Doležel: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Olomouc, Czech Republic

Plant, Soil and Environment, 2007, vol. 53, issue 10, 437-441

Abstract: Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches - i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library - was tested in this work.

Keywords: Cot; CBCS; Cot filtration; BAC library; 1 BS; wheat; genome analysis (search for similar items in EconPapers)
Date: 2007
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:53:y:2007:i:10:id:2195-pse

DOI: 10.17221/2195-PSE

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