Expression of OsNHX1 gene in maize confers salt tolerance and promotes plant growth in the field

M. Chen, Q.-J. Chen, X.-G. Niu, R. Zhang, H.-Q. Lin, C.-Y. Xu, X.-C. Wang, G.-Y. Wang and J. Chen
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M. Chen: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
Q.-J. Chen: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
X.-G. Niu: College of Land and Environment, Shenyang Agricultural University, Shenyang, China
R. Zhang: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
H.-Q. Lin: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
C.-Y. Xu: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
X.-C. Wang: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
G.-Y. Wang: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China
J. Chen: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agriculture University, Beijing, China

Plant, Soil and Environment, 2007, vol. 53, issue 11, 490-498

Abstract: Maize yield is severely affected by soil salinity. In an effort to engineer maize for improved salt tolerance, embryogenic calli of maize were co-bombarded with plasmids containing Oryza sativa Na+/H+ antiporter gene (OsNHX1) and bar genes. For the molecular analysis of putative transgenic samples, PCR, Southern and Northern blots were carried out. The maize plants over-expressing OsNHX1 accumulated more biomass when grown in the presence of 200mM NaCl in greenhouse conditions. Higher Na+ and K+ content was observed in transgenic leaves than in wildtype leaves when treated with 100~200mM NaCl, while the osmotic potential and the proline content in transgenic leaves was lower than in wild-type maize. A field trial revealed that the transgenic maize plants produced higher grain yields than the wild-type plants at the vegetative growth stage. These results demonstrate that the OsNHX1 gene was successfully transferred into Zea mays, and the salt-tolerance of transgenic maize was improved by overexpression of the OsNHX1 gene.

Keywords: Na+/H+ antiporter; salt-tolerance; transgenic maize (search for similar items in EconPapers)
Date: 2007
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:53:y:2007:i:11:id:2302-pse

DOI: 10.17221/2302-PSE

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