Heterologous approach in the search for (candidate) genes

J. Žlůvová
Additional contact information
J. Žlůvová: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Plant, Soil and Environment, 2007, vol. 53, issue 8, 361-364

Abstract: To answer many questions of evolutionary developmental biology, it is necessary to identify genes which could be responsible for the studied traits. In this review, I have summarised the methods that are currently used to identify these genes. Mainly the PCR-based methods of gene cloning are discussed here, with the special attention given to the comparison of primer design strategies and PCR amplification strategies. In addition to the review of methods used by other authors, an overview of our experience with PCR-based gene cloning in dioecious model plant species Silene latifolia is included.

Keywords: evolutionary developmental biology; degenerate primer design; PCR (search for similar items in EconPapers)
Date: 2007
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pse.agriculturejournals.cz/doi/10.17221/2216-PSE.html (text/html)
http://pse.agriculturejournals.cz/doi/10.17221/2216-PSE.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:53:y:2007:i:8:id:2216-pse

DOI: 10.17221/2216-PSE

Access Statistics for this article

Plant, Soil and Environment is currently edited by Kateřina Součková

More articles in Plant, Soil and Environment from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().