The effect of benzothiazolium salt on spruce callus cells

Ľ. Krajnáková, D. Kákoniová, D. Lišková and E. Hlinková
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Ľ. Krajnáková: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
D. Kákoniová: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
D. Lišková: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
E. Hlinková: Institute of Cell Biology, Faculty of Natural Sciences, Comenius University,

Plant, Soil and Environment, 2010, vol. 56, issue 10, 463-469

Abstract: The effect of 3-(benzyloxycarbonylmethyl)-2-benzothiazolinone (SM-550) on the growth parameters, cell division, and cell polymorphism of spruce callus cells (Picea abies L. Karst) was investigated. These results were compared with callus parameters grown on the medium supplemented with NAA. The highest concentration (1 mmol) of SM-550 stimulated the growth process, as well as cell division, shortened the lag-phase, and had a significant effect on cells polymorphism. Its effect was demonstrated especially on long-term culture (3 subcultures - 84 days). On the other hand, the highest stimulation of growth by SM-550 in 1 μmol concentration was determined only in the first subculture. SM-550 in the lowest concentration (1 nmol) used was completely unsuitable in the third subculture, the callus was necrotic and resembled to calli growing on the medium without growth hormones.

Keywords: benzothiazolium salt; callus culture; linear cell polymorphism; Picea abies L. Karst (search for similar items in EconPapers)
Date: 2010
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:56:y:2010:i:10:id:149-2009-pse

DOI: 10.17221/149/2009-PSE

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