Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar test

Alexander Ingo Linn, Pavlína Košnarová, Josef Soukup and Roland Gerhards
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Alexander Ingo Linn: Department of Weed Science, Institute of Phytomedicine, University of Hohenheim, Stuttgart, Germany
Pavlína Košnarová: Department of Agroecology and Biometeorology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Josef Soukup: Department of Agroecology and Biometeorology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Roland Gerhards: Department of Weed Science, Institute of Phytomedicine, University of Hohenheim, Stuttgart, Germany

Plant, Soil and Environment, 2018, vol. 64, issue 8, 386-392

Abstract: Reliable tests on herbicide resistance are important for resistance management. Despite well-established greenhouse bioassays, faster and in-season screening methods would aid in more efficient resistance detection. The feasibility of a chlorophyll fluorescence agar-based test on herbicide resistance in Apera spica-venti L. was investigated. Herbicide resistant and sensitive A. spica-venti seedlings were transplanted into agar containing pinoxaden and pyroxsulam herbicides. Chlorophyll fluorescence was measured and the maximum quantum efficiency of photosystem II (Fv/Fm) was determined 48 h and 72 h after the transplantation to agar, respectively. The Fv/Fm values decreased with increasing herbicide concentration. Dose-response curves and respective ED50 values (herbicide concentration leading to 50% decrease of the Fv/Fm value) were calculated. However, each experiment repetition exhibited different sensitivities of the populations for both herbicides. In certain cases, resistant populations demonstrated similar Fv/Fm values as sensitive populations. Contrary to the findings in Alopecurus myosuroides Huds., discrimination of sensitive and resistant A. spica-venti populations was not feasible. An increased importance of the assessment time due to the herbicide concentrations calibrated for fast responses was assumed in this study.

Keywords: ALS inhibitor; ACCase inhibitor; herbicide-resistant weed; rapid detection; laboratory study; PAM fluorimeter (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:64:y:2018:i:8:id:110-2018-pse

DOI: 10.17221/110/2018-PSE

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