Degradation of trinitrotoluene by transgenic nitroreductase in Arabidopsis plants

Bo Zhu, Hongjuan Han, Xiaoyan Fu, Zhenjun Li, Jianjie Gao and Quanhong Yao
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Bo Zhu: Key Laboratory for the Conservation and Utilization of Important Biological Resources, Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, P.R. China
Hongjuan Han: Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, P.R. China
Xiaoyan Fu: Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, P.R. China
Zhenjun Li: Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, P.R. China
Jianjie Gao: Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, P.R. China
Quanhong Yao: Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, P.R. China

Plant, Soil and Environment, 2018, vol. 64, issue 8, 379-385

Abstract: The explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. TNT is toxic to many organisms, it is known to be a potential human carcinogen, and is persistent in the environment. This study presents a system of phytoremediation by Arabidopsis plants developed on the basis of overexpression of NAD(P)H-flavin nitroreductase (NFSB) from the Sulfurimonas denitrificans DSM1251. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerance and a strikingly higher capacity to remove TNT from their media. The highest specific rate constant of TNT disappearance rate was 1.219 and 2.297 mL/g fresh weight/h for wild type and transgenic plants, respectively. Meanwhile, the nitroreductase activity in transgenic plant was higher than wild type plant. All this indicates that transgenic plants show significantly enhanced tolerances to TNT; transgenic plants also exhibit strikingly higher capabilities of removing TNT from their media and high efficiencies of transformation.

Keywords: nitroaromatic compound; biodegradation; contamination; phytotoxicity; fluorescence (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:64:y:2018:i:8:id:655-2017-pse

DOI: 10.17221/655/2017-PSE

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