Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers

Jana Žiarovská, Lucia Urbanová, Julio Montero-Torres, Adam Kováčik, Lucia Klongová, Rohit Bharati, Sandra Romero-Ortega, Eloy Fernández-Cusimamani and Olga Leuner
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Jana Žiarovská: Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences, Nitra, Slovak Republic
Lucia Urbanová: Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech, Nitra, Slovak Republic
Julio Montero-Torres: Major, Real and Pontifical University of San Francisco Xavier in Chuquisaca, Faculty of Agricultural Sciences, Germplasm Bank - BIORENA, Sucre, Bolivia
Adam Kováčik: Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences, Nitra, Slovak Republic
Lucia Klongová: Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech, Nitra, Slovak Republic
Rohit Bharati: Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Crop Sciences and Agroforestry, Prague, Czech Republic
Sandra Romero-Ortega: Major, Real and Pontifical University of San Francisco Xavier in Chuquisaca, Faculty of Agricultural Sciences, Germplasm Bank - BIORENA, Sucre, Bolivia
Eloy Fernández-Cusimamani: Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Crop Sciences and Agroforestry, Prague, Czech Republic
Olga Leuner: Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Crop Sciences and Agroforestry, Prague, Czech Republic

Plant, Soil and Environment, 2023, vol. 69, issue 12, 615-627

Abstract: Arachis hypogaea L. is an annual legume that is one of the most consumed plant species. On the other hand, it belongs to one of the most monitored clinically important allergens worldwide. The polymorphism of this species based on allergen coding genes could be useful in its characterisation, but previously, no allergen-based marker techniques have been developed for peanuts. A new type of DNA-based markers of coding regions were used to analyse the variability of 21 peanut accessions - BBAP (Bet v1 based amplicon polymorphism), PBAP (profilin based amplicon polymorphism), and VBAP (vicilin based amplicon polymorphism). All of the used technique provided polymorphic fingerprints and distinguished the analysed peanut accessions. The effectivity of these techniques corresponds to the presence of the allergen homologous sequences that are a part of the A. hypogaea genome. VBAP was the most effective in distinguishing the analysed peanut accessions when compared to the results of BBAP and PBAB. For BBAP, two of the analysed accessions provided the same fingerprinting pattern. The ability of the used markers to detect polymorphisms was comparable, with an average polymorphism information content (PIC) value of 0.47.

Keywords: allergen markers; DNA analysis; food safety; genetic resources; peanut accessions (search for similar items in EconPapers)
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:69:y:2023:i:12:id:306-2023-pse

DOI: 10.17221/306/2023-PSE

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