Exogenous proline enhances salt tolerance in wheat: regulating osmolytes, hormonal balance, antioxidant defence, and yield performance

Abeer Hamdy Elhakem
Additional contact information
Abeer Hamdy Elhakem: Department of Biology, College of Sciences and Humanities, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia

Plant, Soil and Environment, 2025, vol. 71, issue 4, 278-292

Abstract: This study investigates the impacts of exogenously applied proline (Pro, 10 mmol/L) on the growth and productivity of wheat plants in saline environments. The findings indicated that increased NaCl concentrations, 60 and 120 mmol/L, further depressed the shoot and root growth parameters and flag leaf area. However, the Pro treatment ameliorated salt stress and improved all growth parameters, reducing the magnitude of such growth inhibitions compared to nontreated plants. It also enhanced the organic osmolyte accumulation, including Pro, total soluble sugars, and total soluble protein, implicated in osmotic balance and cell protection under stress. Furthermore, supplementing Pro improved ionic balance through a reduction in Na accumulation and an enhancement in the uptake of K, Ca, and Mg, thus mitigating the negative effects of salinity on nutrient availability. Pro treatment affected phytohormone levels, especially increasing auxin and gibberellins while decreasing abscisic acid under salt stress. Antioxidant enzymes such as catalase, superoxide dismutase, ascorbate peroxidase, and glutathione reductase, as well as nonenzymatic antioxidants like ascorbic acid and glutathione, were also enhanced by Pro, thereby protecting the plants against oxidative damage. Moreover, it was noticed that Pro treatment substantially improved all yield attributes of wheat plants, such as plant height, spike length, no. of spikelets/main spike, grain no./main spike, grain fresh and dry weights, and grain yield/plant through attenuation of the negative impact of NaCl. In this regard, Pro application appears to be a very promising approach toward mitigating the adversities of salinity in agriculture, especially in crop productivity in saline environments.

Keywords: Triticum aestivum L.; osmotic adjustment; ionic homeostasis; antioxidant enzymes system; phytohormonal regulation; yield optimisation (search for similar items in EconPapers)
Date: 2025
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pse.agriculturejournals.cz/doi/10.17221/97/2025-PSE.html (text/html)
http://pse.agriculturejournals.cz/doi/10.17221/97/2025-PSE.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:71:y:2025:i:4:id:97-2025-pse

DOI: 10.17221/97/2025-PSE

Access Statistics for this article

Plant, Soil and Environment is currently edited by Kateřina Součková

More articles in Plant, Soil and Environment from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().