Enhancing salinity tolerance in rice using Saccharomyces cerevisiae inoculation: physiological and yield responses across diverse genotypes

Heba A. ElSherbiny, Alaa El-Dein Omara, Elsayed E. Gewaily, Walid F. Ghidan, Mahmoud E. Selim, Amany M. Badr, Dalal S. Alshaya, Khadiga Alharbi, Maha Aljabri and Ahmed A.A. Leilah
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Heba A. ElSherbiny: Rice Research Department, Field Crops Research Institute, Agricultural Research Centre, Kafr-Elsheikh, Egypt
Alaa El-Dein Omara: Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, P.R. China
Elsayed E. Gewaily: Rice Research Department, Field Crops Research Institute, Agricultural Research Centre, Kafr-Elsheikh, Egypt
Walid F. Ghidan: Rice Research Department, Field Crops Research Institute, Agricultural Research Centre, Kafr-Elsheikh, Egypt
Mahmoud E. Selim: Rice Research Department, Field Crops Research Institute, Agricultural Research Centre, Kafr-Elsheikh, Egypt
Amany M. Badr: Rice Research Department, Field Crops Research Institute, Agricultural Research Centre, Kafr-Elsheikh, Egypt
Dalal S. Alshaya: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
Khadiga Alharbi: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
Maha Aljabri: Department of Biology, Faculty of Science, Umm Al-Qura University, Makkah, Saudi Arabia
Ahmed A.A. Leilah: Agronomy Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt

Plant, Soil and Environment, 2026, vol. 72, issue 6, 362-379

Abstract: Salinity stress is a major environmental constraint limiting crop productivity worldwide. The application of beneficial microorganisms is an effective strategy to improve plant tolerance to abiotic stresses. This study evaluated the role of Saccharomyces cerevisiae inoculation in enhancing physiological performance, oxidative stress tolerance, and yield of rice genotypes under saline-water irrigation. A lysimeter experiment was conducted during the 2024 and 2025 seasons. Five rice genotypes were exposed to saline water (6 000 ppm) with and without inoculation. Gas exchange, water status, oxidative stress markers, antioxidant enzyme activities, and yield-related traits were assessed. The results showed that inoculation significantly enhanced CO2 assimilation (12.27%), stomatal conductance (15.71%), transpiration rate (8.78%), and relative water content (16.36%) across both seasons. Furthermore, inoculation significantly reduced malondialdehyde (MDA) by 19.75% and hydrogen peroxide (H2O2) by 23.72%. While superoxide dismutase activity (SOD) increased by 35.32% and catalase activity (CAT) by 20.63%. Grain yield per plant improved by 18.91% and biological yield by 12.84%, accompanied by a reduction in grain sterility (14.47%). The assessed genotypes exhibited significant variation across all parameters studied. IRRI-165 and Giza-179 exhibited superior performance and responsiveness. Giza-182 and Sakha-104 displayed intermediate levels, while Giza-177 was the most sensitive genotype. Multivariate analyses confirmed strong positive associations between inoculation and genotypic performance. Whereas genotypic performance was negatively associated with oxidative stress markers. These results suggest that S. cerevisiae inoculation improves rice performance under salinity stress. The enhancement may contribute to the integration of physiological and biochemical mechanisms. Therefore, combining microbial inoculation with tolerant genotypes provides a sustainable strategy to improve rice productivity in salt-affected environments.

Keywords: antioxidant defence; osmotic stress; heatmap and hierarchical clustering; microbial biostimulant; multivariate analysis; oxidative damage; principal component analysis biplot (search for similar items in EconPapers)
Date: 2026
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:72:y:2026:i:6:id:178-2026-pse

DOI: 10.17221/178/2026-PSE

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