Corn poppy (Papaver rhoeas L.) resistance to ALS inhibiting and 2,4-D herbicides in Moroccan and Tunisian rainfed wheat fields

Zakia El-Mastouri, Pavlína Košnarová, Kateřina Hamouzová, Ezzedine Alimi and Josef Soukup
Additional contact information
Zakia El-Mastouri: Department of Agroecology and Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Pavlína Košnarová: Department of Agroecology and Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Kateřina Hamouzová: Department of Agroecology and Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Ezzedine Alimi: Maghreb Phytotest, Tunis, Tunisia
Josef Soukup: Department of Agroecology and Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic

Plant, Soil and Environment, vol. preprint

Abstract: Corn poppy (Papaver rhoeas L.) is one of the most problematic weed species, mainly in rainfed Moroccan and Tunisian cereal crops. The overuse of acetolactate synthase (ALS) inhibiting and/or auxinic herbicides led to the spread of corn poppies resistant to both chemical families in this region. In order to identify and understand the selection drivers of resistance, appropriate characterisation of the resistance profile is necessary. Two experiments were carried out: biological sensitivity tests with ALS inhibiting herbicides (tribenuron-methyl and florasulam) and auxinic herbicides (2,4-d) were carried out with populations sampled in the field where the herbicide failure was observed. Bioassay tests confirmed resistance in all studied populations with an average frequency of 75.13, 30.81, 33.17 and 11.52% with tribenuron, florasulam, 2,4-d and florasulam + 2,4-d, respectively. Corn poppy sampled from both countries exhibited similar frequencies within populations for each tested herbicide. The molecular analysis was conducted with next-generation sequencing (Illumina), allowing massive, precise and rapid sequencing regions of the ALS gene carrying resistance codons. Using this technology, ALS mutant alleles were found in all populations at frequencies ranging from 1.4% to 63.3%, with an average of 16.7%. This study highlights the need to elucidate resistance mechanisms to understand herbicide responses and develop effective strategies for managing resistant corn poppy in rainfed cereals as an essential step to maintain the effectiveness of these molecules as long as possible.

Keywords: broadleaved weeds; weed control; mutation; herbicide resistance (search for similar items in EconPapers)
References: Add references at CitEc
Citations:

Downloads: (external link)
http://pse.agriculturejournals.cz/doi/10.17221/45/2025-PSE.html (text/html)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:preprint:id:45-2025-pse

DOI: 10.17221/45/2025-PSE

Access Statistics for this article

Plant, Soil and Environment is currently edited by Mgr. Kateřina Součková

More articles in Plant, Soil and Environment from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().