Extremely low frequency electromagnetic field generator suitable for plant in vitro studies

Riry Prihatini, Mohamad Puad Abdullah, Tuan Abdul Rashid bin Tuan Abdullah, Ismail Said, Halil Hussin and Norihan Mohamad Saleh
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Riry Prihatini: Indonesian Tropical Fruit Research Institute, Solok, Indonesia
Mohamad Puad Abdullah: Department of Cell and Molecular Biology, Faculty Biotechnology and Biomolecular Sciences, University Putra Malaysia, Selangor DE, Malaysia
Tuan Abdul Rashid bin Tuan Abdullah: Department of Electrical Engineering, Faculty Engineering, University Tenaga Nasional, Selangor DE, Malaysia
Ismail Said: Department of Electrical Engineering, Faculty Engineering, University Tenaga Nasional, Selangor DE, Malaysia
Halil Hussin: Department of Electrical Engineering, Faculty Engineering, University Tenaga Nasional, Selangor DE, Malaysia
Norihan Mohamad Saleh: Department of Cell and Molecular Biology, Faculty Biotechnology and Biomolecular Sciences, University Putra Malaysia, Selangor DE, Malaysia

Research in Agricultural Engineering, 2017, vol. 63, issue 4, 180-186

Abstract: The extremely low frequency electromagnetic field (ELF-EMF) occurs naturally from the earth and artificially as a human invention. The objectives of this study were to develop a suitable ELF-EMF generator for in vitro plants culture studies and to determine the effect of ELF-EMF exposure on in vitro tobacco (Nicotiana tabacum) growth and chlorophyll content. An ELF-EMF generator, the coGEM 1,000 was constructed using four coils of copper wires that were connected to a transformer, multimeter and rheostat. The coGEM 1,000 suitable for tissue culture plants is able to produce stable and uniform 6 and 12 mT 50Hz ELF-EMF in the four coils of the ELF-EMF generator. The tobacco in vitro plantlets were exposed to 6 and 12 mT of 50 Hz ELF-EMF for a period of 0.5, 1, 2 and 4 hours. The exposure to 12 mT ELF-EMF for an hour increased plant growth (shoot height); whereas the exposure to 6 mT Elf-EMF for an hour increased chlorophyll a, chlorophyll b and the total chlorophyll content.

Keywords: chlorophyll content; coGem 1; 000; ELF-EMF; growth; tobacco plant (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlrae:v:63:y:2017:i:4:id:47-2016-rae

DOI: 10.17221/47/2016-RAE

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