Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influx

Z. Sládek, D. Ryšánek and M. Faldyna
Additional contact information
Z. Sládek: Mendel University of Agriculture and Forestry, Brno, Czech Republic
D. Ryšánek: Mendel University of Agriculture and Forestry, Brno, Czech Republic
M. Faldyna: Mendel University of Agriculture and Forestry, Brno, Czech Republic

Veterinární medicína, 2001, vol. 46, issue 7-8, 190-198

Abstract: Distribution of leukocyte types present in virgin bovine mammary glands was analysed in dot plots obtained by flow cytometry (FACS) of samples collected from 10 non-pregnant heifers after induction of leukocyte influx. Changes of percentage of leukocyte types during development and resolution of induced influx in comparison with blood leukocyte pattern allow identification of these cell types on FACS dot plot. The positions of mammary gland granulocyte and lymphocyte regions were identical with those of the corresponding peripheral blood cells. Two basic morphologically distinct types occupying separate regions in dot plots were observed in the population of mononuclear phagocytes (MoP): non-vacuolised monocyte-like macrophages (MoMAC) and vacuolised macrophages (MAC). Influx resolution was characterised by a marked shift of the MoMAC region towards that of MAC recognisable in dot plots by a separate region of intermediate MoP forms. The study provides a pattern of dynamics of percentages of mammary gland leukocyte types during influx development and resolution as imaged by FACS.

Keywords: flow cytometry; bovine virgin mammary gland; induced influx (search for similar items in EconPapers)
Date: 2001
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/7882-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/7882-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:46:y:2001:i:7-8:id:7882-vetmed

DOI: 10.17221/7882-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().