An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli

D.Y. Zhang, J.Y. Zhou, J. Fang, J.Q. Hu, J.X. Wu and A.X. Mu
Additional contact information
D.Y. Zhang: College of Life Science,
J.Y. Zhou: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,
J. Fang: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,
J.Q. Hu: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,
J.X. Wu: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine,
A.X. Mu: General Station of Veterinary Medicine, Hangzhou Bureau of Agriculture, Hangzhou, China

Veterinární medicína, 2005, vol. 50, issue 8, 336-344

Abstract: The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in Chinawas expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the N-ELISA to evaluate the antibody level of chicken serum. In an experiment to test field samples for antibody detection, the N-ELISA assay shared 95.7% identity of total positive ratio with the commercial ELISA kit. It indicated that the N-ELISA assay, which was safer and easier to prepare than traditional methods, was a good candidate for evaluation of IB vaccine efficiency and virus exposure.

Keywords: infectious bronchitis virus; nucleocapsid protein; in vitro expression; ELISA (search for similar items in EconPapers)
Date: 2005
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5632-vetmed

DOI: 10.17221/5632-VETMED

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