Appearance of iron-labeled blood mononuclear cells in electron microscopy

D. Horky, I. Lauschova, M. Klabusay, M. Doubek, P. Sheer, S. Palsa and J. Doubek
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D. Horky: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
I. Lauschova: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
M. Klabusay: Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic
M. Doubek: Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic
P. Sheer: Department of Physiology and Pathophysiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic
S. Palsa: Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic
J. Doubek: Department of Physiology and Pathophysiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic

Veterinární medicína, 2006, vol. 51, issue 3, 89-92

Abstract: Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes.

Keywords: ultrastructure; iron nanoparticles; cultivation; animal model (search for similar items in EconPapers)
Date: 2006
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5525-vetmed

DOI: 10.17221/5525-VETMED

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