Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals

A. Pauciullo, S. Kubickova, H. Cernohorska, K. Petrova, D. Di Berardino, L. Ramunno and J. Rubes
Additional contact information
A. Pauciullo: Department of Animal Science and Food Inspection, University of Naples "Federico II", via Portici (NA), Italy
S. Kubickova: Veterinary Research Institute, Brno, Czech Republic
H. Cernohorska: Veterinary Research Institute, Brno, Czech Republic
K. Petrova: Veterinary Research Institute, Brno, Czech Republic
D. Di Berardino: Department of Animal Science and Food Inspection, University of Naples "Federico II", via Portici (NA), Italy
L. Ramunno: Department of Animal Science and Food Inspection, University of Naples "Federico II", via Portici (NA), Italy
J. Rubes: Veterinary Research Institute, Brno, Czech Republic

Veterinární medicína, 2006, vol. 51, issue 5, 224-231

Abstract: In this study, new classes of tandemly repeated DNA sequences in the centromeric regions of three farm animal species are reported. Pericentromeric regions of bovine chromosome X, porcine chromosome 14 and equine chromosome 1 were microdissected by laser and amplified by DOP-PCR. Painting probes showed strong hybridization signals on their corresponding centromeres. Specific DNA fragments were isolated by cloning and sequenced. For each species, high homology level was found among the sequences. The presence of specific repeats within each sequence allows us to consider them as monomeric units or parts of longer tandemly repeated monomeric units that generate a specific higher-order repeat. In this respect, the isolation of new centromeric sequences enriches the genetic map of these three species and represents a useful tool for FISH analysis, thus expanding our knowledge on centromeres of farm animal species.

Keywords: Bos taurus; Sus scrofa; Equus caballus; cattle; pig; horse; centromeres; tandem repeats; chromosomes; FISH (search for similar items in EconPapers)
Date: 2006
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/5541-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/5541-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:51:y:2006:i:5:id:5541-vetmed

DOI: 10.17221/5541-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().