Effect of polychlorinated biphenyls on the secretion of oxytocin from luteal and granulosa cells in cow: possible involvement of glucocorticoid receptors

J.J. Mlynarczuk and J. Kotwica
Additional contact information
J.J. Mlynarczuk: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
J. Kotwica: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland

Veterinární medicína, 2006, vol. 51, issue 7, 391-398

Abstract: Polychlorinated biphenyls (PCBs) stimulate oxytocin secretion from bovine granulosa and luteal cells. Since oxytocin on the one hand is released from ovarian cells by cortisol and on the other hand PCBs can be bound by glucocorticoid receptors (GCr), we have tested the hypothesis that PCBs acting via GCr can stimulate oxytocin secretion. In preliminary studies the effect of RU486 (GCr blocker) on cells viability was tested. Thereafter, a selected dose of RU486 (105M), which did not affect cell viability, was used in further experiments. It appears that this dose of RU486 completely blocked GCr against cortisol-stimulated oxytocin secretion, in both types of cells. Furthermore, granulosa cells (105) from follicles of two sizes (>1 cm < in diameter) and luteal cells (105) from day 5-10 of estrous cycle were incubated for 72 h with congeners of PCB (126, 77 or 153) at doses of 1, 10 or 100 ng/ml each, separately or jointly with RU486. The effect of PCB 77 and 153 on oxytocin secretion was blocked by RU486, but it did not change the effect evoked by PCB126 in both granulosa and luteal cells. We assume that some PCB congeners can affect oxytocin secretion from granulosa and luteal cells acting via GCr.

Keywords: polychlorinated biphenyls; oxytocin; glucocorticoid receptors; ovary; cow (search for similar items in EconPapers)
Date: 2006
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/5559-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/5559-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:51:y:2006:i:7:id:5559-vetmed

DOI: 10.17221/5559-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().