Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism

A. Kozubska-Sobocinska and B. Rejduch
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A. Kozubska-Sobocinska: National Research Institute of Animal Production, Balice n. Krakow, Poland
B. Rejduch: National Research Institute of Animal Production, Balice n. Krakow, Poland

Veterinární medicína, 2008, vol. 53, issue 5, 250-254

Abstract: The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring.

Keywords: sheep; leukocyte chimerism; FISH; spermatozoa; heterosomes (search for similar items in EconPapers)
Date: 2008
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1855-vetmed

DOI: 10.17221/1855-VETMED

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