Detection methods for Mycobacterium avium subsp paratuberculosis in milk and milk products: a review

I. Slana, F. Paolicchi, B. Janstova, P. Navratilova and I. Pavlik
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I. Slana: Veterinary Research Institute, Brno, Czech Republic
F. Paolicchi: National Institute of Agricultural Technology, Balcarce, Argentina
B. Janstova: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
P. Navratilova: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
I. Pavlik: Veterinary Research Institute, Brno, Czech Republic

Veterinární medicína, 2008, vol. 53, issue 6, 283-306

Abstract: Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis, a disease with considerable economic impact, principally on dairy cattle herds. Animals with paratuberculosis shed viable MAP especially in their milk, faeces and semen. MAP may have a role in the development of Crohn's disease in humans via the consumption of contaminated milk and milk products. The current methods of milk pasteurization are not sufficient to kill all MAP cells present in milk and MAP has been cultured from raw or pasteurized milk and isolated from cheese. The purpose of the present study was to review the different methods used for detection of MAP in milk and milk products. We analyze the current methods for direct or non direct identification of MAP and culture and molecular biology methods that can be applied to milk and milk products.

Keywords: PCR; cultivation; IS900; ELISA; MELISA; food safety; potential zoonosis; Johne's disease (search for similar items in EconPapers)
Date: 2008
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Citations: View citations in EconPapers (3)

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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:53:y:2008:i:6:id:1859-vetmed

DOI: 10.17221/1859-VETMED

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