Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic

R. Smitalova, L. Rodak, B. Smid and I. Psikal
Additional contact information
R. Smitalova: Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic
L. Rodak: Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic
B. Smid: Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic
I. Psikal: Department of Bacteriology and Virology, Veterinary Research Institute, Brno, Czech Republic

Veterinární medicína, 2009, vol. 54, issue 1, 12-18

Abstract: Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rotaviruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain.

Keywords: group B rotavirus; group C rotavirus; nongroup A rotavirus; enteritis; RT-PCR; semi-nested PCR (search for similar items in EconPapers)
Date: 2009
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/3081-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/3081-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:54:y:2009:i:1:id:3081-vetmed

DOI: 10.17221/3081-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().