Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells

M.K. Kowalik, D. Slonina and J. Kotwica
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M.K. Kowalik: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
D. Slonina: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
J. Kotwica: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland

Veterinární medicína, 2009, vol. 54, issue 5, 205-214

Abstract: Progesterone (P2 (PGES), (b) protein expression of COX-2, PGFS and PGES, and (c) P4 receptor membrane component 1 (PGRMC1) gene expression in bovine endometrial cells. The epithelial endometrial cells (2.5 × 105/ml) from Days 14-16 of the oestrous cycle were incubated for 72-96 h to attach the cells to the bottom of a well. Next, the cells were preincubated for 30 min with P4 and P5 (10-5M each) and incubated for 4 h and 6 h alone or with OT (10-7M). Thereafter, the medium was collected for PGE2 and PGFM determination, while cells were harvested for gene and protein expression analysis. The used steroids: (a) inhibited OT-stimulated PGF2α, but not PGE2 secretion from endometrial cells, (b) did not affect the expression of mRNA for COX-2, PGFS, PGES and PGRMC1 in endometrial cells after 4 and 6 h, (c) they decreased OT-stimulated COX-2 mRNA expression only after 6 h incubation, and (d) did not influence COX-2, PGFS and PGES protein expression after 6 h. These results indicate that P4 and P5 inhibit OT-stimulated secretion/production of luteolytic PGF2α by a transcription-independent mechanism and partly by down-regulation of COX-2 mRNA.

Keywords: non-genomic effect; progesterone; progesterone membrane receptor component 1; endometrium; bovine (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:54:y:2009:i:5:id:58-2009-vetmed

DOI: 10.17221/58/2009-VETMED

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