Pathology of subclinical paratuberculosis (Johne's Disease) in Awassi sheep with reference to its occurrence in Jordan

N.Q. Hailat, W. Hananeh, A.S. Metekia, J.R. Stabel, A. Al-Majali and S. Lafi
Additional contact information
N.Q. Hailat: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan
W. Hananeh: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan
A.S. Metekia: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan
J.R. Stabel: USDA-ARS, National Animal Disease Centre, Ames, USA
A. Al-Majali: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan
S. Lafi: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan

Veterinární medicína, 2010, vol. 55, issue 12, 590-602

Abstract: In this study, the pathological lesions and occurrence of subclinical Johne's disease in Awassi sheep is investigated using histopathological (HP) and immunohistochemical (IHC) examinations, Acid Fast Staining (AFS) of tissue sections, direct smears from tissues and culture. Histopathological examination of 202 ilea and the corresponding mesenteric lymph nodes (179) was conducted. In addition, IHC examination, using rabbit polyclonal antiserum, of 134 ilea and 123 mesenteric lymph nodes was also conducted. The occurrence of the disease was observed in 50% and 93% of the ilea examined using histopathology and IHC techniques, respectively. Fifty nine percent of lymph nodes were positive by IHC. The histopathological lesions were graded from І-IV, I being the least severe, based on the type of cellular infiltrate (lymphocytes, macrophages and epithelioid cells) and the severity of the lesions. Grades III and IV (SP) were considered positive while I and II were considered suspected. Analysis of the results also revealed that the majority of suspected cases (grades I and II) reacted positive with the IHC. Furthermore, the IHC reactions were classified into three categories depending on the number of stained cells and the intensity of the staining (I-mild, II-moderate and III-strong). Direct smears, and tissue sections obtained from the ilea and stained with AFS revealed that out of 202 tissue samples, 53 (26%) and 22 (11%) were positive, respectively. Results of the culture revealed that 22 (11%) out of 202 were positive. These results showed that the occurrence of paratuberculosis (Johne's disease) in Awassi sheep is very high in Jordan and needs further study in order to develop rational methods of control effective for the Jordanian sheep population.

Keywords: sheep; paratuberculosis; histopathology; acid fast stain; ileum; immunohistochemistry (search for similar items in EconPapers)
Date: 2010
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/2947-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/2947-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2947-vetmed

DOI: 10.17221/2947-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().