TLR agonists activate HPV11 E7-pulsed DCs to promote a specific T cell response in a murine model

X.H. Mao, X.Z. Chen, W.W. Zhang, J.Y. Wang, L.F. Liu, Qiankun Zhou, K.J. Zhu and H. Cheng
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X.H. Mao: Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
X.Z. Chen: Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China
W.W. Zhang: Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
J.Y. Wang: Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
L.F. Liu: Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
K.J. Zhu: Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China
H. Cheng: Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Veterinární medicína, 2011, vol. 56, issue 12, 602-611

Abstract: : Some TLR agonists may up-regulate the activation of dendritic cells caused by viral antigenic peptides and antigen-specific cytotoxic T lymphocytes, which are crucial in HPV vaccine development. We investigated the ability of three TLR agonists, imiquimod, PIC and CpG, to stimulate the maturation of murine BM-DCs loaded with HPV11E7 CTL epitopes, and the subsequent effect on HPV-specific T cell responses and tumour protection in a C57BL/6 mouse model. We found that TLR agonists, mostly PIC and imiquimod, stimulated the maturation of BM-DCs pulsed with HPV11E7 CTL epitope peptide. In combination with the epitope peptide, the TLR agonists CPG and PIC augmented epitope-specific Th1 cytokine production in vivo, while imiquimod and CPG, but not PIC, enhanced Th1 cytokine production in vitro. However, we failed to observe in vivo CTL cytotoxicity and anti-tumour protection upon TLR ligation in our mouse model. Our results demonstrate that TLR agonists activate HPV11E7 CTL epitope pulsed BM-DCs to promote specific Th1 immunity in C57BL/6 mouse model, indicating the promise of TLR agonists as adjuvants for HPV epitope/DC-based multifaceted vaccines against HPV infections such as condyloma accuminatum.

Keywords: HPV - human papillomavirus; TLR - toll-like receptor; DC - dendritic cell (search for similar items in EconPapers)
Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:56:y:2011:i:12:id:4438-vetmed

DOI: 10.17221/4438-VETMED

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