Immunohistological changes in skin wounds during the early periods of healing in a rat model

F. Sabol, L. Dancakova, P. Gal, T. Vasilenko, M. Novotny, K. Smetana and L. Lenhardt
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F. Sabol: Pavol Jozef Safarik University, Kosice, Slovak Republic
L. Dancakova: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic
P. Gal: East-Slovak Institute of Cardiovascular Diseases, Kosice, Slovak Republic
T. Vasilenko: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic
M. Novotny: Pavol Jozef Safarik University, Kosice, Slovak Republic
K. Smetana: Charles University, Prague, Czech Republic
L. Lenhardt: University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic

Veterinární medicína, 2012, vol. 57, issue 2, 77-82

Abstract: The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin 14, α-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

Keywords: tissue repair and regeneration; cell differentiation; transition; proliferation; wound healing (search for similar items in EconPapers)
Date: 2012
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:57:y:2012:i:2:id:5253-vetmed

DOI: 10.17221/5253-VETMED

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