Prevalence of mastitis pathogens in milk from clinically healthy cows

D. Cervinkova, H. Vlkova, I. Borodacova, J. Makovcova, V. Babak, A. Lorencova, I. Vrtkova, D. Marosevic and Z. Jaglic
Additional contact information
D. Cervinkova: Veterinary Research Institute, Brno, Czech Republic
H. Vlkova: Veterinary Research Institute, Brno, Czech Republic
I. Borodacova: Veterinary Research Institute, Brno, Czech Republic
J. Makovcova: Veterinary Research Institute, Brno, Czech Republic
V. Babak: Veterinary Research Institute, Brno, Czech Republic
A. Lorencova: Veterinary Research Institute, Brno, Czech Republic
I. Vrtkova: Laboratory of Agrogenomics, Mendel University, Brno, Czech Republic
D. Marosevic: Veterinary Research Institute, Brno, Czech Republic
Z. Jaglic: Veterinary Research Institute, Brno, Czech Republic

Veterinární medicína, 2013, vol. 58, issue 11, 567-575

Abstract: A total of 669 individual cow milk samples originating from asymptomatic cows from 16 dairy farms were examined for the presence of microorganisms with the potential to cause mastitis. Coagulase-negative staphylococci clearly predominated (53.5% positive samples) followed by streptococci and enterococci (both occurring in 16.1% samples). Among streptococci, so-called mastitis streptococci (S. uberis, S. dysgalactiae and S. agalactiae) prevailed (11.7% positive samples). Enterobacteriaceae were found in 10.0% samples, most of which (6.6% samples) were positive for Escherichia coli. Yeasts (mainly Candida spp.) were found in 8.2% samples. One of the major mastitis pathogens, Staphylococcus aureus subsp. aureus, was isolated from 9.0% of samples. S. aureus isolates were further characterised in terms of their capability to form biofilm, antimicrobial susceptibility and clonality (PFGE). All S. aureus isolates were capable of biofilm formation and were generally susceptible to the majority of tested antibiotics. The exception was ampicillin, resistance to which was observed in 27.7% isolates. Therefore, the relatively frequent occurrence of S. aureus could be attributed to persistent intramammary infections due to biofilm formation rather than low efficacy of particular antibiotics. PFGE analysis revealed clonal spread of certain S. aureus isolates within and between farms indicating that certain lineages of S. aureus mastitis strains are particularly successful.

Keywords: mastitis; bovine; intramammary; IMI; etiology; epidemiology; macrorestriction (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:58:y:2013:i:11:id:7138-vetmed

DOI: 10.17221/7138-VETMED

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