O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in Korea

J.W. Byun, B.Y. Jung, H.Y. Kim, J.M. FairbrotheR, M.H. Lee and W.K. Lee
Additional contact information
J.W. Byun: Animal, Plant and Fisheries Quarantine Inspection Agency, Anyang, Republic of Korea
B.Y. Jung: Animal, Plant and Fisheries Quarantine Inspection Agency, Anyang, Republic of Korea
H.Y. Kim: Animal, Plant and Fisheries Quarantine Inspection Agency, Anyang, Republic of Korea
J.M. FairbrotheR: Faculty of Veterinary Medicine, University of Montreal, Sicotte, Saint-Hyacinthe, Quebec, Canada
M.H. Lee: Animal, Plant and Fisheries Quarantine Inspection Agency, Anyang, Republic of Korea
W.K. Lee: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea

Veterinární medicína, 2013, vol. 58, issue 9, 468-476

Abstract: A total of 116 Escherichia (E.) coli isolates isolated from neonatal diarrhoeic piglets were serogrouped and tested for the presence of virulence genes for fimbrial and non-fimbrial adhesins, intimin, and enterotoxins. Pulsed-field gel electrophoresis (PFGE) pulsotypes were also analyzed within O149 enterotoxigenic E. coli (ETEC) isolates. In total, Sixty eight (58.6%) isolates were serotyped. Among them, forty three (63.2%) belonged to 12 serogroups in the descending order: O149, O8, O157, O101, O60, O9, O117, O127, O138, O167, O27 and O97. The predominant pathotype was ETEC (68, 58.6%) which is closely associated with F4 (37, 31.9%) and LT:STb:EAST1 (23, 19.8%) out of the isolates harbouring at least one gene for toxin and/or fimbria. Among non-fimbrial adhesins, porcine attaching and effacing-associated factor (paa) was closely associated with F4-positive isolates (64.7%) rather than F18-positive isolates (5.9%). Adhesion involved in diffuse adherence (AIDA) was only detected in 3 isolates. No eae-positive isolates were detected. The PFGE pattern of 15 O149 isolates was grouped into 12 pulsotypes at 88% similarity level. The results show a wide variety of distinct restriction patterns though all belonged to the same serogroup O149. It is believed that a broad array of O serogroup and virulence genes are associated with neonatal diarrhoea in Korea.

Keywords: Escherichia coli; piglet; O-serogroup; pathotype; virulence gene (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:58:y:2013:i:9:id:7031-vetmed

DOI: 10.17221/7031-VETMED

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