Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis

Milanjeet, Harkirat Singh, N.K. Singh, N.D. Singh, Chanchal Singh and S.S. Rath
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Milanjeet: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Harkirat Singh: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
N.K. Singh: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
N.D. Singh: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Chanchal Singh: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
S.S. Rath: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

Veterinární medicína, 2014, vol. 59, issue 3, 129-136

Abstract: Evaluation of blood samples collected from 214 dogs from Ludhiana, Punjab (India) was performed for the presence of Ehrlichia canis using PCR-based assays targeting a portion of the 16S rRNA gene. Of the total samples subjected to routine blood smear examination, the morulae of E. canis were detected in 2.34% samples. Nested PCR assay produced amplicons of expected size (389 bp) specific for E. canis in 41.59% (89/214) of samples. The results of multivariate analysis showed that the prevalence of E. canis was higher in the summer as compared to the winter season (P = 0.031) and in dogs younger than six-month-old as compared to older dogs (P < 0.001), while breed and sex of the host were not significantly associated with the occurrence of the disease.

Keywords: canine monocytic ehrlichiosis; Ehrlichia canis; haematological abnormalities; nested PCR; risk factors (search for similar items in EconPapers)
Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:59:y:2014:i:3:id:7380-vetmed

DOI: 10.17221/7380-VETMED

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