Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine

L. Ondruska, V. Parkanyi, J. Vasicek, R. Jurcik, E. Hanusova, D. Vasicek, A. Balazi and F. Vizzarri
Additional contact information
L. Ondruska: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic
V. Parkanyi: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic
J. Vasicek: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic
R. Jurcik: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic
E. Hanusova: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic
D. Vasicek: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic
A. Balazi: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Luzianky, Slovak Republic
F. Vizzarri: University of Molise, Campobasso, Italy

Veterinární medicína, 2016, vol. 61, issue 10, 571-576

Abstract: The aim of this study was to evaluate the acute phase reaction and immune response of rabbits triggered by vaccination with a live attenuated myxoma virus (MXT) vaccine. Thirteen adult and 11 juvenile New Zealand white rabbit-based crossbreed rabbits, were used. Samples of rabbit peripheral blood were collected from vena auricularis centralis into heparinised tubes before vaccination and 48 h after vaccination. All animals were vaccinated by subcutaneous injection (0.5 ml) with a MXT vaccine. The blood plasma C-reactive protein level was measured by an ELISA kit using a double-antibody sandwich. For phenotyping of lymphocytes the fresh cells were stained with the following anti-rabbit monoclonal antibodies: anti-IgM, anti-CD4, anti-CD8 and anti-pan T2. Our results show that the use of attenuated myxoma virus vaccine significantly decreases the level of C-reactive protein in blood plasma of adult rabbits by 38.14% (P < 0.05) and of juvenile rabbits by 37.63% (P < 0.001), within 48 h. The rabbit C-reactive protein after MXT vaccination is a negative acute phase protein. In the group of adult rabbits the immune response to MXT vaccination was accompanied by a non-significant decrease in CD4+, pT2+, IgM+ subsets. On the other hand the values of CD8+, CD4+CD8+ and CD4+/CD8+ were non-significantly higher after MXT vaccination.

Keywords: rabbit; Oryctolagus cuniculus; myxomatosis; ELISA (search for similar items in EconPapers)
Date: 2016
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/159/2015-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/159/2015-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:61:y:2016:i:10:id:159-2015-vetmed

DOI: 10.17221/159/2015-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().