Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity

H. Akkari, F. B'chir, S. Hajaji, M. Rekik, E. Sebai, H. Hamza, M.A. Darghouth and M. Gharbi
Additional contact information
H. Akkari: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia
F. B'chir: Institut National de Recherche et d'Analyse Physico-Chimique-Pole Technologique de Sidi Thabet, Sidi Thabet, Tunisia
S. Hajaji: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia
M. Rekik: International Center for Agricultural Research in the Dry Areas (ICARDA), Amman, Jordan
E. Sebai: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia
H. Hamza: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia
M.A. Darghouth: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia
M. Gharbi: National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia

Veterinární medicína, 2016, vol. 61, issue 6, 308-316

Abstract: Capparis spinosa is cultivated for several proprieties and the different parts of the plant (flower buds, fruits, leaf and seeds) have been employed in drugs, foods and cosmetics. This study aimed to explore the antioxidant potential effect and to assess the anthelmintic efficacy of an aqueous extract of Capparis spinosa. In vitro antioxidant activity of aqueous extracts from the fresh leaves and flower buds of C. spinosa was measured by determining free radical-scavenging activity against DPPH and ABTS radical cations. In vitro anthelmintic activities against eggs and adult worms of Haemonchus contortus from sheep were investigated in comparison to albendazole. DPPH and ABTS activities of both aqueous extracts increased in a dose dependent manner. The IC50 values of aqueous extracts from fresh leaves, flowers buds and ascorbic acid in the DPPH radical scavenging assay were 101.40; 70.40 and 57.56 μg/ml, respectively. The IC50 values of aqueous extracts fresh leaves, flowers buds and ascorbic acid in the ABTS radical scavenging assay were 110; 87.89 and 58.17 μg/ml, respectively. Both plant extracts showed ovicidal activity at all tested concentrations. The aqueous extract from flower buds (IC50 = 2.76 mg/ml) showed higher inhibitory effects (P < 0.05) than aqueous extract from leaves (IC50 = 8.54 mg/ml) in the egg hatching assay. The aqueous extract from flower buds inhibited more worms than the aqueous extract from leaves at all tested concentrations. After 6 h of exposure, the highest tested concentrations of aqueous extracts from flower buds and leaves induced 100 and 41.66% inhibition of motility, respectively. These results show for the first time that C. spinosa possesses in vitro anthelmintic properties which may be related to its high content of phenolic compounds such as flavonoids and tannins.

Keywords: Capparis spinosa; free radical-scavenging ability; anthelmintic; Haemonchus contortus (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/169/2015-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/169/2015-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:61:y:2016:i:6:id:169-2015-vetmed

DOI: 10.17221/169/2015-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().