Ultrastructural and molecular identification of Sarcocystis tenella (Protozoa, Apicomplexa) in naturally infected Korean native goats

E.J. Hong, C. Sim, J.S. Chae, H.C. Kim, J. Park, K.S. Choi, D.H. Yu, C.H. Park, J.G. Yoo and B.K. Park
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E.J. Hong: College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea
C. Sim: Department of Biology, Baylor University, Waco, Texas, USA
J.S. Chae: Laboratory of Veterinary Internal Medicine, Research Institute and BK21 Program for Veterinary Science, Seoul, Republic of Korea
H.C. Kim: College of Veterinary Medicine, Gangwon National University, Gangwon, Republic of Korea
J. Park: College of Veterinary Medicine, Chonbuk National University, Chonbuk, Republic of Korea
K.S. Choi: College of Environmental Science, Kyungbook National University, Kyungbook, Republic of Korea
D.H. Yu: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea
C.H. Park: Veterinary Service Laboratory, South branch of Gangwondo, Republic of Korea
J.G. Yoo: Laboratory of Veterinary Clinics, National Institute of Animal Science Rural Development Administration, Republic of Korea
B.K. Park: College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea

Veterinární medicína, 2016, vol. 61, issue 7, 374-381

Abstract: Sheep is the intermediate host of the protozoan parasite Sarcocystis tenella, while the dog is its definitive host. This study was conducted to determine the prevalence of natural infection with S. tenella in slaughtered Korean native goat, Capra hircus coreanae, in the Republic of Korea. H-E stained heart tissues were investigated for the presence of sarcocysts. Of the 103 goats, three (2.91%) were diagnosed as positive for S. tenella by light, electron microscopic and molecular examination. The histopathological study showed a low frequency of microscopic Sarcocystis infection in slaughtered goats. In transmission electron microscopy, the sarcocysts were confirmed as S. tenella. Further DNA sequencing and phylogenic analysis support our identification of S. tenella with a 18S rRNA sequence identity of 100% between the experimental sequence and S. tenella. To our knowledge, this is the first record of S. tenella in Korean native goats from Korea. We thus report that the domestic goat is another intermediate host for S. tenella.

Keywords: Capra hircus coreanae; cardiac muscle; Sarcocystis tenella; sarcocysts; bradyzoites; ultrastructure; molecular biology (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:61:y:2016:i:7:id:93-2015-vetmed

DOI: 10.17221/93/2015-VETMED

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