EconPapers    
Economics at your fingertips  
 

Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR

O. Melter, P. Svec, J. Tkadlec, J. Doskar, H. Kinska and R. Pantucek
Additional contact information
O. Melter: 2nd Medical Faculty, Charles University, Prague, Czech Republic
P. Svec: Faculty of Science, Masaryk University, Brno, Czech Republic
J. Tkadlec: 2nd Medical Faculty, Charles University, Prague, Czech Republic
J. Doskar: Faculty of Science, Masaryk University, Brno, Czech Republic
H. Kinska: Laboratory of Veterinary Medicine (Labvet), Prague, Czech Republic
R. Pantucek: Faculty of Science, Masaryk University, Brno, Czech Republic

Veterinární medicína, 2017, vol. 62, issue 2, 81-89

Abstract: Staphylococcus pseudintermedius is a genuine opportunistic pathogen of the skin, especially in canids. However, characterisation of virulence, antimicrobial resistance and genotypic variability in methicillin-susceptible S. pseudintermedius isolates has not been fully explored. In this study, coagulase-positive staphylococcal isolates collected from dogs of various breeds and ages suffering from dermatitis (n = 70), pyoderma (n = 7), and otitis (n = 7), from districts of Prague (Czech Republic) and surrounding areas, were characterised using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and repetitive sequence-based PCR fingerprinting. Susceptibility to antimicrobial agents was determined, virulence factor genes for leukocidin (lukSF-I), exfoliatins (exi, expB, and siet), enterotoxin C (seccanine) and enterotoxin-related genes (se-int and sel) were detected using multiplex PCR and the genotypes of S. pseudintermedius isolates were determined using SmaI macrorestriction analysis. The majority of the staphylococcal isolates (n = 84) were identified as S. pseudintermedius (n = 79) and all of them were susceptible to methicillin/oxacillin (MSSP). About half of the strains (n = 41) were resistant to macrolide-lincosamide-streptogramin B antimicrobial agents and resistance was mediated in all but one of the strains by the erm(B) gene. The genes for lukSF-I, siet, se-int, and sel were detected in the majority of the MSSP strains (96.2%, 100%, 100%, and 73.4%, respectively). Investigated canine S. pseudintermedius isolates were highly heterogeneous, which prevented the correlation of any specific lineage to a particular infection, dog breed, or region of origin.

Keywords: Staphylococcus pseudintermedius; macrolide lincosamide-streptogramin B (MLSB) resistance; genotyping; virulence genes (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/105/2016-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/105/2016-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:62:y:2017:i:2:id:105-2016-vetmed

DOI: 10.17221/105/2016-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().

 
Page updated 2025-03-19
Handle: RePEc:caa:jnlvet:v:62:y:2017:i:2:id:105-2016-vetmed