Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco

Tae Geum Kim, Bong Jo Kang, Sang Ik Park and Tae Jung Kim
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Tae Geum Kim: Center for Jeongup Industry-Academy-Institute Cooperation, Chonbuk National University, Jeollabuk-do, Republic of Korea
Bong Jo Kang: Ocean and Fisheries Research Institute, Jeju special self-governing province, Republic of Korea
Sang Ik Park: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea
Tae Jung Kim: College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea

Veterinární medicína, 2019, vol. 64, issue 10, 456-461

Abstract: The viral haemorrhagic septicaemia virus (VHSV) causes high mortality in many marine and freshwater fish species, resulting in heavy economic losses in fish farming. Previously, cholera toxin B subunit (CTB)-fused recombinant viral haemorrhagic septicaemia virus glycoproteins (rec-VHSV-GPs) have been successfully expressed in tobacco, Nicotiana benthamiana. Here, we evaluated the potential of rec-VHSV-GPs as an oral vaccine against a live viral challenge. After immunisation of mice and fish (olive flounder, Paralichthys olivaceous) with those antigenic proteins in a feed additive form, the antibody titres were increased statistically, especially in the primed groups (P < 0.0001) in both the mouse and fish. After the viral challenge under low water temperature culture conditions (below 18 °C), the immunised fish were protected successfully against the challenge, showing a significantly lower mortality rate (P < 0.05). This result suggests that this plant-based immunisation system could induce an effective immune response. It could be used as a candidate to develop an oral vaccine for fish.

Keywords: flounder; oral vaccine; plant expression; viral haemorrhagic septicaemia virus (search for similar items in EconPapers)
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:64:y:2019:i:10:id:26-2019-vetmed

DOI: 10.17221/26/2019-VETMED

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