Development and application of a low-priced duplex quantitative PCR assay based on SYBR Green I for the simultaneous detection of porcine deltacoronavirus and porcine sapelovirus

Lu Sj, Ma My, Yan Xg, Zhao Fj, Hu Wy, Ding Qw, Ren Hj, Xiang Yq and Zheng Ll
Additional contact information
Lu Sj: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Ma My: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Yan Xg: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Zhao Fj: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Hu Wy: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Ding Qw: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Ren Hj: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Xiang Yq: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China
Zheng Ll: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P.R. China

Veterinární medicína, 2023, vol. 68, issue 3, 106-115

Abstract: Porcine deltacoronavirus (PDCoV) and porcine sapelovirus (PSV) are two viruses that can cause diarrhoea in pigs and bring great economic loss to the pig industry. In this research, a duplex real-time quantitative polymerase chain reaction (qPCR) assay based on SYBR Green І was developed to simultaneously detect PDCoV and PSV. No specific melting peaks were found in other porcine diarrhoea-associated viruses, indicating that the method developed in this study had good specificity. The detection limits of PDCoV and PSV were 1.0 × 101 copies μl-1 and 1.0 × 102 copies μl-1, respectively. The duplex real-time qPCR assay tested two hundred and three (203) intestinal and faecal samples collected from diarrhoeal and asymptomatic pigs. The positive rates of PDCoV and PSV were 20.2% and 23.2%, respectively. The co-infection rate of PDCoV and PSV was 13.8%. To evaluate the accuracy of the developed method, conventional PCR and singular TaqMan real-time qPCR assays for PDCoV/PSV were also used to detect the samples. The results showed that the duplex real-time qPCR assay was consistent with the singular assays, but its sensitivity was higher than conventional PCR methods. This duplex real-time qPCR assay provides a rapid, sensitive and reliable method in a clinic to simultaneously detect PDCoV and PSV.

Keywords: duplex real-time qPCR; PDCoV; PSV (search for similar items in EconPapers)
Date: 2023
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://vetmed.agriculturejournals.cz/doi/10.17221/79/2022-VETMED.html (text/html)
http://vetmed.agriculturejournals.cz/doi/10.17221/79/2022-VETMED.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlvet:v:68:y:2023:i:3:id:79-2022-vetmed

DOI: 10.17221/79/2022-VETMED

Access Statistics for this article

Veterinární medicína is currently edited by Ing. Helena Smolová Ph.D.

More articles in Veterinární medicína from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().