Curcumin promote autophagy via miR-582-5p and Akt/mTOR axis

Huang, Mendjargal, Odkhuu, Boldbaatar, Guo and Baldandorj

Health Leadership and Quality of Life, 2025, vol. 4, 628

Abstract: Objective: This study aimed to investigate the role of Curcumin in modulating miR-582-5p expression in cisplatin (DDP)-resistant cervical cancer cells. Methods: miR-582-5p expression levels were quantified by qPCR. Cell viability following DDP treatment was assessed using the CCK-8 assay. Colony formation ability was evaluated, and the expression of autophagy-related proteins (ATG7, Beclin-1, LC3-II/I) as well as the phosphorylation status of Akt and mTOR were analyzed by Western blotting. Results: miR-582-5p expression was downregulated in DDP-resistant tissues and in Hela/DDP and SiHa/DDP cells. Curcumin treatment reduced the IC50 values of both cell lines and suppressed colony formation. Curcumin upregulated ATG7, Beclin-1, and LC3-II/I expression in DDP-resistant Hela and SiHa cells, while concurrently inhibiting the phosphorylation of Akt and mTOR. miR-582-5p modulated Akt phosphorylation and influenced Curcumin-induced autophagy in these resistant cell lines. Additionally, the phosphorylation status of Akt affected the autophagic response to Curcumin. Conclusions: Curcumin enhances autophagy in DDP-resistant cervical cancer cells, accompanied by downregulation of miR-582-5p and inhibition of Akt/mTOR phosphorylation.

Date: 2025
References: Add references at CitEc
Citations:

There are no downloads for this item, see the EconPapers FAQ for hints about obtaining it.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:dbk:health:v:4:y:2025:i::p:628:id:628

DOI: 10.56294/hl2025628

Access Statistics for this article

More articles in Health Leadership and Quality of Life from AG Editor
Bibliographic data for series maintained by Javier Gonzalez-Argote ().